Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: Sequences of the qPCR primers.

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Sequencing

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: SiRNA sequences.

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques:

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: (A) Immunofluorescent localization of FoxO3 in turbot liver. (a) DAPI staining; (b) FoxO3 staining; (c) Merge. (B) Expressions of foxo3 in different tissues of turbot. The results were expressed as the mean ± standard deviation (n = 3). Bars bearing the different letters are significantly different between tissues by one-way analysis of variance (ANOVA) ( P < 0.05).

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Staining, Standard Deviation

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: (A) Relative level of foxo3 mRNA in primary cultured hepatocytes treated with si foxo3 . Results are represented as mean ± standard deviation (n = 3). Values with different letters mean significant differences ( P < 0.05). (B) The OD values of MTT. Results are represented as mean ± standard deviation (n = 3). Values with different letters mean significant differences ( P < 0.05).

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Cell Culture, Standard Deviation

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: Relative mRNA levels of foxo3 , tnf-α , il-1β , il-6 , nf-κb , myd88 , cd83 , jnk and tlr-2 in primary hepatocytes of turbot. The results are expressed as the mean ± standard deviation (n = 4). ‘*’ means significantly different between two group ( P < 0.05). The different letters are significantly different ( P < 0.05).

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Standard Deviation

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: Relative mRNA expression of foxo3 , tnf-α , il-1β , il-6 , nf-κb , myd88 , cd83 , jnk , tlr-2 in turbot liver. The results are expressed as the mean ± standard deviation (n = 4). ‘*’ means significantly different between two group ( P < 0.05). The different letters are significantly different ( P < 0.05).

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Expressing, Standard Deviation

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: Relative mRNA levels of foxo3 , tnf-α , il-1β , il-6 , nf-κb , myd88 , cd83 , jnk and tlr-2 in the turbot primary hepatocytes. The results are expressed as the mean ± standard deviation (n = 4). ‘*’ means significantly different between two group ( P < 0.05). The different letters are significantly different ( P < 0.05).

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Standard Deviation

Journal: Frontiers in Immunology

Article Title: FoxO3 Modulates LPS-Activated Hepatic Inflammation in Turbot ( Scophthalmus maximus L.)

doi: 10.3389/fimmu.2021.679704

Figure Lengend Snippet: Relative mRNA levels of foxo3 , tnf-α , il-1β , il-6 , nf-κb , myd88 , cd83 , jnk , tlr-2 in turbot liver. The results are expressed as the mean ± standard deviation (n = 4). ‘*’ means significantly different between two group ( P < 0.05). The different letters are significantly different ( P < 0.05).

Article Snippet: In brief, a piece of liver tissue was taken out from a turbot, then washed with PBS and fixed in 4% paraformaldehyde at room temperature for 30 min. Then, it was blocked for 1 h in 3% BSA, and followed by incubation with specific primary monoclonal rabbit anti-human antibody FoxO3 (1:200, AF609, Beyotime, China) (diluted with PBS appropriately) overnight at 4°C.

Techniques: Standard Deviation